Hello. I have an image (grayscale) of spots, that have a spread of intensities and sizes.

I have the centroid location of each spot and an "size" of each spot obtained by my own encircled energy calculation for both x&y directions.

`data = 8.35 8.14 43.00 2266.00 10.76 8.24 43.00 2362.00 8.96 11.07 43.00 2459.00 8.55 7.98 43.00 2555.00`

where

`col 1 is the size of my object in y col 2 is the size in xcol 3 is the x centroid locationcol 4 is the y centroid location`

What I am trying to achieve is at each x,y centroid location, draw a circle with a diameter given by the average of the size in x & size in y, and then caluclate the integrated intensity in the circle. The circles (representing the size) may be different for each spot.

As a second request, I would also like to then consider a distance d, say 15 pixels from the x,y centroid location and obtain the background values from a square or circle that I can call background to then subtract from the integrated signal requested above to get the background subtracted integrated signal.

Thanks for any help Jason

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