I'm doing some image processing for biology research. I'm looking at images of a larva that bends into different shapes as it moves around. I'm able to binarize the images and automatically locate the head and tail. However, I need a way to draw a line between the head and the tail that follows the body of the larva, so I can calculate its curvature.

So the data is a 640 x 480 matrix of zeros, with an elongated and curved field of ones somewhere in the middle that represents the larva. In addition there are two points [A, B] located at either end of the field of ones, which correspond to head and the tail. How can I get Matlab find for example the shortest distance along the ones between A and B? (It doesn't have to be the shortest distance, it's just one way of constraining the question.) A polynomial or spline won't do because the larva often bends 360 degrees or more.

Thanks!! Chris

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